CD44 antibody (Biotin)

Catalog No. ABIN94124

The Mouse Monoclonal anti-CD44 antibody has been validated for FACS, WB, ELISA and IP. It is suitable to detect CD44 in samples from Human. There are 3+ publications available.

Quick Overview for CD44 antibody (Biotin) (ABIN94124)

Target: CD44

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: MEM-85

Product Details anti-CD44 Antibody (Biotin)

Purpose: Anti-Hu CD44 Biotin

Specificity: The antibody MEM-85 reacts with an extracellular antigen of both cell surface-expressed and soluble form of CD44 antigen (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.), it is negative on platelets and hepatocytes.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogen: Leukocytes of a patient suffering from LGL Type Leukaemia.

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-3 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD44 antibody (Biotin) (ABIN94124)

Kolar, Mehta, Pelayo, Capra: "A novel human B cell subpopulation representing the initial germinal center population to express AID." in: Blood, Vol. 109, Issue 6, pp. 2545-52, (2007) (PubMed).

Bazil, Strominger: "Metalloprotease and serine protease are involved in cleavage of CD43, CD44, and CD16 from stimulated human granulocytes. Induction of cleavage of L-selectin via CD16." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1314-22, (1994) (PubMed).

Stefanová, Hilgert, Bazil, Kristofová, Horejsí: "Human leucocyte surface glycoprotein CDw44 and lymphocyte homing receptor are identical molecules." in: Immunogenetics, Vol. 29, Issue 6, pp. 402-4, (1989) (PubMed).

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: CD44 Molecule,CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.,PGP-I, HUTCH-I, ECMR-III, Hermes antigen, Hyaluronate receptor, Heparan sulfate proteoglycan, Epican, MC56, MIC4, INLU, LHR

Gene ID: 960

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling