CD46 Molecule, Complement Regulatory Protein (CD46) antibody (FITC)

Details for Product No. ABIN94152
Antigen
Reactivity
Human, Cow (Bovine)
273
48
32
31
21
7
Host
Mouse
203
124
2
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
33
27
20
13
10
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
201
174
100
66
60
59
33
16
14
10
8
8
5
2
2
1
Options
Immunogen HPB-ALL human T cell line
Clone MEM-258
Isotype IgG1
Specificity The antibody MEM-258 recognizes an epitope on SCR4 (the membrane-proximal SCR) domain of CD46 (Membrane cofactor protein). CD46 is 56-66 kDa dimeric transmembrane protein expressed on T and B lymphocytes, platelets, monocytes, granulocytes, endothelial cells, epithelial cells and fibroblast, it is negative on erythrocytes.
Cross-Reactivity (Details) Human, Bovine
Antigen
Alternative Name CD46 (CD46 Antibody Abstract)
Background CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b complement fragments, regulates T cell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing (CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor for several human pathogens (both bacteria and viruses), and its ligation alteres T lymphocyte polarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function. CD46 is a protector of placental tissue and is also expressed on the inner acrosomal membrane of spermatozoa.
Pathways Regulation of Actin Filament Polymerization
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD46 Molecule, Complement Regulatory Protein (CD46) antibody (FITC) (ABIN94152) Surface staining of human peripheral blood cells with anti-CD46 (MEM-258) FITC.
Product cited in: Kälin, Amstutz, Gastaldelli, Wolfrum, Boucke, Havenga, DiGennaro, Liska, Hemmi, Greber: "Macropinocytotic uptake and infection of human epithelial cells with species B2 adenovirus type 35." in: Journal of virology, Vol. 84, Issue 10, pp. 5336-50, 2010 (PubMed).

Wang, Tuve, Erdman, Lieber: "Receptor usage of a newly emergent adenovirus type 14." in: Virology, Vol. 387, Issue 2, pp. 436-41, 2009 (PubMed).

Fleischli, Sirena, Lesage, Havenga, Cattaneo, Greber, Hemmi: "Species B adenovirus serotypes 3, 7, 11 and 35 share similar binding sites on the membrane cofactor protein CD46 receptor." in: The Journal of general virology, Vol. 88, Issue Pt 11, pp. 2925-34, 2007 (PubMed).

Loré, Adams, Havenga, Precopio, Holterman, Goudsmit, Koup: "Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 3, pp. 1721-9, 2007 (PubMed).

Fremeaux-Bacchi, Moulton, Kavanagh, Dragon-Durey, Blouin, Caudy, Arzouk, Cleper, Francois, Guest, Pourrat, Seligman, Fridman, Loirat, Atkinson: "Genetic and functional analyses of membrane cofactor protein (CD46) mutations in atypical hemolytic uremic syndrome." in: Journal of the American Society of Nephrology : JASN, Vol. 17, Issue 7, pp. 2017-25, 2006 (PubMed).

Weyand, Lee, Higashi, Cawley, Yoshihara, So: "Monoclonal antibody detection of CD46 clustering beneath Neisseria gonorrhoeae microcolonies." in: Infection and immunity, Vol. 74, Issue 4, pp. 2428-35, 2006 (PubMed).

Fleischli, Verhaagh, Havenga, Sirena, Schaffner, Cattaneo, Greber, Hemmi: "The distal short consensus repeats 1 and 2 of the membrane cofactor protein CD46 and their distance from the cell membrane determine productive entry of species B adenovirus serotype 35." in: Journal of virology, Vol. 79, Issue 15, pp. 10013-22, 2005 (PubMed).