CD63 antibody

Catalog No. ABIN94214

The Mouse Monoclonal anti-CD63 antibody has been validated for FACS, IHC (p), IP and ICC. It is suitable to detect CD63 in samples from Human. There are 3+ publications available.

Quick Overview for CD63 antibody (ABIN94214)

Target: CD63

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD63 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: MEM-259

Product Details anti-CD63 Antibody

Purpose: Anti-Hu CD63 Purified

Specificity: The antibody MEM-259 reacts with an extracellular/luminal epitope of CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: HPB-ALL T cell line

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 2 μg/mL, positive material: activated platelets, neutrophils and basophils.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: spleen.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD63 antibody (ABIN94214)

Sørensen, Holemans, van Veen, Martin, Arslan, Haagendahl, Holen, Hamouda, Eggermont, Palmgren, Vangheluwe: "Parkinson disease related ATP13A2 evolved early in animal evolution." in: PLoS ONE, Vol. 13, Issue 3, pp. e0193228, (2018) (PubMed).

Heneberg, Riegerová, Kučera: "Pimecrolimus Is a Potent Inhibitor of Allergic Reactions to Hymenopteran Venom Extracts and Birch Pollen Allergen In Vitro." in: PLoS ONE, Vol. 10, Issue 11, pp. e0142953, (2015) (PubMed).

Cerny, Feng, Yu, Miyake, Borgonovo, Klumperman, Meldolesi, McNeil, Kirchhausen: "The small chemical vacuolin-1 inhibits Ca(2+)-dependent lysosomal exocytosis but not cell resealing." in: EMBO reports, Vol. 5, Issue 9, pp. 883-8, (2004) (PubMed).

Target Details for CD63

Target: CD63

Alternative Name: CD63

Background: CD63 Molecule,CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.,OMA81H, Granulophysin, Tetraspanin-30, Tspan-30, MLA1, ME491, LAMP-3, OMA81H, TSPAN30

Gene ID: 967

UniProt: P08962