CD72 antibody

Catalog No. ABIN94227

Cited in 1 publication. The Mouse Monoclonal anti-CD72 antibody (Clone 3F3) (ABIN94227) specifically detects CD72 in FACS and IP. The antibody is reactive with Human samples.

Quick Overview for CD72 antibody (ABIN94227)

Target: CD72 (CD72 Molecule (CD72))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD72 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 3F3

Product Details anti-CD72 Antibody

Purpose: Anti-Hu CD72 Purified

Specificity: The mouse monoclonal antibody 3F3 recognizes an extracellular epitope of CD72, a 39-43 kDa type II membrane glycoprotein (C-type lectin family). CD72 is a pan-B cell marker expressed throughout the B lymphocytes diferentiation with the exception of plasma cells, it is also present on follicular dendritic cells.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Normal human lymphocytes from a lymph node.

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD72 antibody (ABIN94227)

Mizrahi, Markel, Porgador, Bushkin, Mandelboim: "CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72." in: PLoS ONE, Vol. 2, Issue 9, pp. e818, (2007) (PubMed).

Target Details for CD72

Target: CD72 (CD72 Molecule (CD72))

Alternative Name: CD72

Background: CD72 Molecule,CD72 is a transmembrane glycoprotein expressed as a homodimer especially in B cells, but also in other antigen presenting cells such as dendritic cells and macrophages. Through one of its immunoreceptor tyrosine-based inhibitory motives (ITIMs), CD72 interacts with tyrosine phosphatase SHP-1, thereby suppressing B cell responsiveness. Binding of CD72 with its ligand CD100 (Sema4D) prevents BCR association and phosphorylation of CD72 and results in dissociation of SHP-1 from CD72, thus enables B cell activation.,LYB2

Gene ID: 971

UniProt: P21854

Pathways: BCR Signaling