CD9 antibody

Catalog No. ABIN94242

This anti-CD9 antibody is a Mouse Monoclonal antibody detecting CD9 in FACS, WB and IP. Suitable for Human and Cow. This Primary Antibody has been cited in 5+ publications.

Quick Overview for CD9 antibody (ABIN94242)

Target: CD9

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD9 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Clone: IVA50

Product Details anti-CD9 Antibody

Purpose: Anti-Bov CD9 Purified

Specificity: The antibody IVA50 reacts with an extracellular epitope of CD9 antigen (bovine), a 24 kDa single transmembrane polypeptide expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes. This antibody crossreacts with CD9 on human platelets.

Cross-Reactivity (Details): Human, Bovine

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Bovine thrombocytes.

Isotype: IgG2

Application Details

Application Notes: Western blotting: Recommended dilution: 1 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD9 antibody (ABIN94242)

de Pontes, Altei, Galan, Bilić, Guillemin, Kuleš, Horvatić, Ribeiro, de Paula, Pereira, Lucheis, Mrljak, Eckersall, Ferreira, Dos Santos: "Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes." in: The journal of venomous animals and toxins including tropical diseases, Vol. 26, pp. e20190067, (2020) (PubMed).

Tomásková, Dusinský, Horovská, Simon: "A set of monoclonal antibodies specific for bovine cell surface molecule CD9." in: Folia biologica, Vol. 45, Issue 5, pp. 225-6, (2000) (PubMed).

Mateo, Perez de la Lastra, Moreno, Dusinsky, Bilka, Simon, Horovska, Llanes: "Biochemical characterization of antigens detected with anti-platelet monoclonal antibodies." in: Veterinary immunology and immunopathology, Vol. 52, Issue 4, pp. 363-70, (1997) (PubMed).

Naessens, Howard, Hopkins: "Nomenclature and characterization of leukocyte differentiation antigens in ruminants." in: Immunology today, Vol. 18, Issue 8, pp. 365-8, (1997) (PubMed).

Sopp, Howard: "Investigation of PC36 (BoCD45R)." in: Veterinary immunology and immunopathology, Vol. 52, Issue 4, pp. 271-4, (1997) (PubMed).

Target Details for CD9

Target: CD9

Alternative Name: CD9

Background: CD9 Molecule,CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.,MIC3, MRP-1, BTCC-1, DRAP-27, TSPAN29

Gene ID: 280746

UniProt: P30932

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization