CD99 antibody (FITC)

Catalog No. ABIN94260

This anti-CD99 antibody is a Mouse Monoclonal antibody detecting CD99 in FACS. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for CD99 antibody (FITC) (ABIN94260)

Target: CD99

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD99 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: MEM-131

Product Details anti-CD99 Antibody (FITC)

Purpose: Anti-Hu CD99R FITC

Specificity: The antibody MEM-131 reacts with CD99R, an extracellular epitope restricted to a subset of CD99 Molecule expressed on myeloid cells, NK cells and T lymphocytes.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: HPB-ALL human peripheral blood leukemia T-cell line

Isotype: IgM

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD99 antibody (FITC) (ABIN94260)

Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, (2002) (PubMed).

Olweus, Lund-Johansen, Terstappen: "CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells." in: Blood, Vol. 85, Issue 9, pp. 2402-13, (1995) (PubMed).

Target Details for CD99

Target: CD99

Alternative Name: CD99R

Background: CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.,MIC2, HBA71, blood group Xg