HLA-DR1 antibody

Catalog No. ABIN94350

This anti-HLA-DR1 antibody is a Mouse Monoclonal antibody detecting HLA-DR1 in FACS, ELISA and WB. Suitable for Human.

Quick Overview for HLA-DR1 antibody (ABIN94350)

Target: HLA-DR1 (MHC Class II HLA-DR1 (HLA-DR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DR1 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Western Blotting (WB)

Clone: MEM-267

Product Details anti-HLA-DR1 Antibody

Purpose: Anti-HLA-DR1 (empty) Purified

Specificity: The antibody MEM-267 specifically binds extracellularly to the empty but not peptide-loaded form of HLA-DR1. DR is the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: The antibody MEM-267 stains immature dendritic cells that express empty cell surface MHC molecules, but not cells that express predominantly peptide loaded forms.
Western blotting: Recommended dilution: 1 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for HLA-DR1

Target: HLA-DR1 (MHC Class II HLA-DR1 (HLA-DR1))

Alternative Name: HLA-DR1

Background: HLA-DR1 belongs to the HLA class II beta chain paralogues. The MHC Class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. MHC Class II molecules are expressed in antigen presenting cells (APC). The beta chain is approximately 26-28 kDa. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.