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IgE antibody

This anti-IgE antibody is a Mouse Monoclonal antibody detecting IgE in ELISA and FACS. Suitable for Human. This Primary Antibody has been cited in 2+ publications.
Catalog No. ABIN94398

Quick Overview for IgE antibody (ABIN94398)

Target

See all IgE Antibodies
IgE

Reactivity

  • 24
  • 3
  • 2
  • 1
Human

Host

  • 17
  • 10
  • 2
  • 1
Mouse

Clonality

  • 22
  • 7
  • 1
Monoclonal

Conjugate

  • 21
  • 3
  • 2
  • 2
  • 1
  • 1
This IgE antibody is un-conjugated

Application

  • 18
  • 9
  • 8
  • 5
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
ELISA, Flow Cytometry (FACS)

Clone

4G7-325
  • Purpose

    Anti-Hu IgE Purified

    Specificity

    The mouse monoclonal antibody 4G7.325 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4H10 antibodies to IgE. The epitope is located within the amino acids 103-115 (WSDYNFDYSSSEE).

    Cross-Reactivity (Details)

    Human

    Purification

    Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

    Purity

    > 95 % (by SDS-PAGE)

    Immunogen

    Purified human IgE.

    Isotype

    IgG
  • Application Notes

    Flow cytrometry: Recommended dilution: 1-4 μg/mL.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Do not freeze.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C. Do not freeze.
  • Smith, Benjamin, Hozic, Derewenda, Smith, Thomas, Gafvelin, van Hage-Hamsten, Chapman: "The molecular basis of antigenic cross-reactivity between the group 2 mite allergens." in: The Journal of allergy and clinical immunology, Vol. 107, Issue 6, pp. 977-84, (2001) (PubMed).

    Mueller, Smith, Chapman, Rule, Benjamin: "Hydrogen exchange nuclear magnetic resonance spectroscopy mapping of antibody epitopes on the house dust mite allergen Der p 2." in: The Journal of biological chemistry, Vol. 276, Issue 12, pp. 9359-65, (2001) (PubMed).

  • Target

    IgE

    Background

    Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E

    Molecular Weight

    180 kDa
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