Ig Light Chains antibody
Quick Overview for Ig Light Chains antibody (ABIN94401)
Target
Reactivity
Host
Clonality
Conjugate
Application
Clone
-
-
Purpose
- Anti-Bov Ig Light Chains Purified
-
Specificity
- The antibody IVA285-1 reacts with bovine Ig (light chains). Ig light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
-
Cross-Reactivity (Details)
- Bovine, Sheep
-
Purification
- Purified by protein-A affinity chromatography.
-
Purity
- > 95 % (by SDS-PAGE)
-
Immunogen
- Fraction of Bovine immunoglobulin.
-
Isotype
- IgG1
-
-
-
-
Application Notes
-
Western blotting: Recommended dilution: 0,5-1 μg/mL, overnight at 4 °C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12 % separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM.
Flow cytometry: Recommended dilution: 1-4 μg/mL -
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- 1 mg/mL
-
Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
-
Preservative
- Sodium azide
-
Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Handling Advice
- Do not freeze.
-
Storage
- 4 °C
-
Storage Comment
- Store at 2-8°C. Do not freeze.
-
-
-
Monoclonal antibody to the light chain of bovine immunoglobulin." in: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, (2006) (PubMed).
: "
-
Monoclonal antibody to the light chain of bovine immunoglobulin." in: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, (2006) (PubMed).
-
- Ig Light Chains
-
Background
- Immunoglobulin light chains
Target
-