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Mouse anti-Human IgM Antibody

ELISA, FACS, WB, ICC Monoclonal CH2 IgG1 kappa unconjugated
Catalog No. ABIN94402

Quick Overview for Mouse anti-Human IgM Antibody (ABIN94402)

Target

IgM

Clonality

  • 548
  • 102
Monoclonal

Application

  • 391
  • 296
  • 295
  • 256
  • 183
  • 145
  • 91
  • 91
  • 64
  • 62
  • 54
  • 53
  • 47
  • 38
  • 18
  • 17
  • 10
  • 6
  • 5
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC)

Clone

CH2

Isotype

IgG1 kappa
  • Reactivity

    • 232
    • 167
    • 92
    • 42
    • 34
    • 34
    • 30
    • 24
    • 17
    • 14
    • 13
    • 13
    • 13
    • 13
    • 12
    • 4
    • 2
    • 2
    Human

    Host

    • 373
    • 241
    • 91
    • 22
    • 14
    • 5
    • 4
    • 1
    • 1
    Mouse

    Purpose

    Anti-Hu IgM Purified

    Specificity

    The antibody CH2 reacts with Fc fragment of human IgM.

    Cross-Reactivity (Details)

    Human

    Purification

    Purified by protein-A affinity chromatography.

    Purity

    > 95 % (by SDS-PAGE)

    Immunogen

    Purified human IgM.
  • Application Notes

    Western blotting: Recommended dilution: 1 μg/mL.
    Flow cytometry: Recommended dilution: 1-4 μg/mL, extracellular and intracellular staining.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Do not freeze.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C. Do not freeze.
  • Target

    IgM

    Target Type

    Antibody

    Background

    Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.,immunoglobulin M

    Molecular Weight

    900 kDa
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