Acrosin antibody

Catalog No. ABIN94455

This anti-Acrosin antibody is a Mouse Monoclonal antibody detecting Acrosin in WB, FACS and ICC. Suitable for Pig. This Primary Antibody has been cited in 7+ publications.

Quick Overview for Acrosin antibody (ABIN94455)

Target: Acrosin (ACR)

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Acrosin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: ACR-2

Product Details anti-Acrosin Antibody

Purpose: Anti-Por Acrosin Purified

Specificity: The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.

No Cross-Reactivity: Cow, Dog, Human

Cross-Reactivity (Details): Porcine

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-12 μg/mL. Intracellular staining.
Immunocytochemistry: Membrane permeabilization (acetone) is essential.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-Acrosin antibody (ABIN94455)

Ded, Dostalova, Zatecka, Dorosh, Komrskova, Peknicova: "Fluorescent analysis of boar sperm capacitation process in vitro." in: Reproductive biology and endocrinology : RB&E, Vol. 17, Issue 1, pp. 109, (2020) (PubMed).

Ded, Dostalova, Dorosh, Dvorakova-Hortova, Peknicova: "Effect of estrogens on boar sperm capacitation in vitro." in: Reproductive biology and endocrinology : RB&E, Vol. 8, pp. 87, (2010) (PubMed).

Peknicova, Capkova, Geussova, Ivanova, Mollova: "Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro." in: Theriogenology, Vol. 56, Issue 2, pp. 211-23, (2001) (PubMed).

Moos, Peknicova, Tesarik: "Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction." in: Biology of reproduction, Vol. 49, Issue 2, pp. 408-15, (1993) (PubMed).

Moos, Peknicova, Tesarik: "Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction." in: Biochimica et biophysica acta, Vol. 1176, Issue 3, pp. 199-207, (1993) (PubMed).

Moos, Tesarik, Leca, Peknicova: "Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin." in: FEBS letters, Vol. 294, Issue 1-2, pp. 27-30, (1992) (PubMed).

Peknicová, Moos: "Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test." in: Andrologia, Vol. 22, Issue 5, pp. 427-35, (1991) (PubMed).

Target Details for Acrosin

Target: Acrosin (ACR)

Alternative Name: Acrosin

Background: Acrosin,Acrosin is a serine proteinase expressed in the acrosome of mature spermatozoa. This enzyme facilitates penetration of the sperm through the zona pellucida of the ovum.,ACR, FBP53

Gene ID: 397098

UniProt: P08001

Pathways: cAMP Metabolic Process