Acrosin antibody (AA 201-300)

Catalog No. ABIN1387665

Product Details anti-Acrosin Antibody

Target: Acrosin (ACR)

Binding Specificity: AA 201-300

Reactivity: Human, Rat, Mouse, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acrosin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Goat, Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Acrosin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Acrosin antibody (ABIN1387665)

Deng, Zhang, Yu, Wang, Chen, Han, Cheng, Lian, Liu et al.: "Melatonin up-regulates the expression of the GATA-4 transcription factor and increases testosterone secretion from Leydig cells through RORα signaling in an in vitro goat spermatogonial stem cell ..." in: Oncotarget, Vol. 8, Issue 66, pp. 110592-110605, (2017) (PubMed).

Deng, Chen, Wang, Zhang, Tang, Li, Wang, Cheng, Jin, Li, Zhang, Yu, Lian, Liu, Liu: "Melatonin promotes development of haploid germ cells from early developing spermatogenic cells of Suffolk sheep under in vitro condition." in: Journal of pineal research, Vol. 60, Issue 4, pp. 435-47, (2016) (PubMed).

Liang, Cheng, Jiang, He, Wang, Jiang, Hou, Li, Liu, Wang: "The noncircadian function of the circadian Clock gene in the regulation of male fertility." in: Journal of biological rhythms, Vol. 28, Issue 3, pp. 208-17, (2013) (PubMed).

Target Details for Acrosin

Target: Acrosin (ACR)

Alternative Name: Acrosin (ACR Products)

Synonyms: ACR antibody, MGC130972 antibody, Acro antibody, Acrosin antibody, acrosin antibody, acrosin L homeolog antibody, acrosin prepropeptide antibody, ACR antibody, acr.L antibody, Acr antibody

Background:

Synonyms: Acrosin, ACR, ACRS

Background: Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.

Gene ID: 49

UniProt: P10323

Pathways: cAMP Metabolic Process