TRIM antibody (Intracellular)

Catalog No. ABIN94489

This Mouse Monoclonal antibody specifically detects TRIM in WB, IP and ICFC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for TRIM antibody (Intracellular) (ABIN94489)

Target: TRIM (TRAT1) (T Cell Receptor Associated Transmembrane Adaptor 1 (TRAT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRIM antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Intracellular Flow Cytometry (ICFC)

Clone: TRIM-04

Product Details anti-TRIM Antibody

Binding Specificity: AA 29-186, Intracellular

Purpose: Anti-TRIM Purified

Specificity: The antibody TRIM-04 recognizes an intracellular epitope of T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.

No Cross-Reactivity: Mouse

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Recombinant intracellular domain (aa 29-186) of human TRIM.

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL, intracellular staining.
Western blotting: Non-reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-TRIM antibody (ABIN94489)

Kirchgessner, Dietrich, Scherer, Isomäki, Korinek, Hilgert, Bruyns, Leo, Cope, Schraven: "The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain." in: The Journal of experimental medicine, Vol. 193, Issue 11, pp. 1269-84, (2001) (PubMed).

Target Details for TRIM

Target: TRIM (TRAT1) (T Cell Receptor Associated Transmembrane Adaptor 1 (TRAT1))

Alternative Name: TRIM

Background: T cell receptor associated transmembrane adaptor 1,TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.,TRAT, TCRIM

Gene ID: 50852

UniProt: Q6PIZ9

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway