Acid Phosphatase antibody

Catalog No. ABIN94564

Supplier Product No.: 200-4140-0100

The Rabbit Polyclonal anti-Acid Phosphatase antibody has been validated for WB, ELISA and IHC. It is suitable to detect Acid Phosphatase in samples from Potato.

Quick Overview for Acid Phosphatase antibody (ABIN94564)

Target: Acid Phosphatase (ACP)

Reactivity: Potato

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acid Phosphatase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-Acid Phosphatase Antibody

Supplier Product No.: 200-4140-0100

Supplier: Rockland

Purpose: Acid Phosphatase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato].

Characteristics: Synonyms: rabbit anti-Acid Phosphatase Antibody, Acid phosphatase 1 soluble antibody, PAP3, Acid phosphatase of erythrocyte antibody, Adipocyte acid phosphatase antibody, Cytoplasmic phosphotyrosyl protein phosphatase antibody, HAAP antibody

Purification: Anti-Acid Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Acid Phosphatase [Potato]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Acid Phosphatase has been tested by western blot and is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:4,000 - 1:40,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Acid Phosphatase

Target: Acid Phosphatase (ACP)

Alternative Name: Acid Phosphatase

Background: Background: The catalytic activity of Acid Phosphatase is phosphate monoester + H2O = an alcohol + phosphate.

UniProt: Q6J5M8