AKT1 antibody (pThr308)
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- Target See all AKT1 Antibodies
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
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Binding Specificity
- pThr308
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This AKT1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Dot Blot (DB)
- Supplier Product No.
- 600-401-269
- Supplier
- Rockland
- Purpose
- AKT phospho T308 Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
- Characteristics
- Synonyms: rabbit anti-AKT pT308 Antibody, AKT1 phospho T308, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
- Purification
- AKT phospho T308 Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: Anti-AKT pT308 polyclonal antibody was produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to residues surrounding threonine 308 of human AKT1 protein.
Immunogen Type: Conjugated Peptide
- Isotype
- IgG
- Top Product
- Discover our top product AKT1 Primary Antibody
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- Application Notes
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Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: 1:200
Application Note: AKT antibody is phospho specific for pT308 and is tested for western blotting, Immunohistochemistry, dot blot, and ELISA. This antibody is suitable for flow cytometry.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:15,000
Other: DB: 5 μg/mL
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.97 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store AKT phospho T308 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
- Alternative Name
- AKT1 (AKT1 Products)
- Background
- Background: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.
- Gene ID
- 207, 62241011
- UniProt
- P31749
- Pathways
- PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect
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