AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1)

Details for Product anti-AKT1 Antibody No. ABIN94785
Antigen
Reactivity
Human
1247
705
592
52
52
48
33
33
30
28
6
6
5
4
4
3
3
2
1
1
1
1
Host
Rabbit
1049
233
9
4
2
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
62
37
34
32
29
26
19
19
19
18
18
18
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
1063
594
563
204
177
149
116
61
55
33
26
18
13
11
8
7
5
3
3
2
1
1
1
1
1
Options
Immunogen Anti-AKT phospho T308 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to aa 302-311 of human AKT protein conjugated to KLH.
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Antigen
Alternative Name AKT (AKT1 Antibody Abstract)
Background AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at  Ser 473 and Thr 308.  AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways.  AKT Exhibits tight control over cell proliferation and cell viability.  Overexpression or inappropriate activation of AKT is noted in many types of cancer.   AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.  AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
Gene ID 207, 62241011
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling
Application Notes AKT antibody is phospho specific for pT308 and is suitable for western blotting and ELISA.
Restrictions For Research Use only
Format Liquid
Concentration 0.97 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody (ABIN94785) Western Blot of Rabbit anti-Akt phospho T308 antibody. Lane 1: GST tagged AKT1 un-act...