D Amino Acid Oxidase antibody

Catalog No. ABIN94968

Supplier Product No.: 200-601-143-0100

The Sheep Polyclonal anti-D Amino Acid Oxidase antibody has been validated for WB, ELISA and IP. It is suitable to detect D Amino Acid Oxidase in samples from Pig.

Quick Overview for D Amino Acid Oxidase antibody (ABIN94968)

Target: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Reactivity: Pig

Host: Sheep

Clonality: Polyclonal

Conjugate: This D Amino Acid Oxidase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-D Amino Acid Oxidase Antibody

Supplier Product No.: 200-601-143-0100

Supplier: Rockland

Purpose: D-Amino Acid Oxidase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified D-Amino Acid Oxidase [Pig Kidney].

Characteristics: Synonyms: sheep anti-D-Amino Acid Oxidase Antibody, D-amino-acid oxidase, DAMOX, DAAO, DAO

Purification: Anti-D-AMINO ACID OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: D-Amino Acid Oxidase [Pig Kidney]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: D-AMINO ACID OXIDASE antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of D-Amino Acid Oxidase [Pig Kidney] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for D Amino Acid Oxidase

Target: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Alternative Name: DAO

Background: Background: D-Amino Acid Oxidase regulates the level of the neuromodulator D-serine in the brain. It has high activity towards D-DOPA and contributes to dopamine synthesis. It could act as a detoxifying agent which removes D-amino acids accumulated during aging. It acts on a variety of D-amino acids, with a preference for those having small hydrophobic side chains, followed by those bearing polar, aromatic, and basic groups. D-Amino Acid Oxidase does not act on acidic amino acids.

Gene ID: 397134

NCBI Accession: NP_999231

UniProt: P00371