beta-Amylase 1 antibody

Catalog No. ABIN95010

Supplier Product No.: 200-4147-0100

This anti-beta-Amylase 1 antibody is a Rabbit Polyclonal antibody detecting beta-Amylase 1 in WB and ELISA. Suitable for Sweet potato.

Quick Overview for beta-Amylase 1 antibody (ABIN95010)

Target: beta-Amylase 1 (BAM1)

Reactivity: Sweet potato

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta-Amylase 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-beta-Amylase 1 Antibody

Supplier Product No.: 200-4147-0100

Supplier: Rockland

Purpose: Beta Amylase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified b Amylase [Sweet Potato].

Characteristics: Synonyms: rabbit anti-Beta Amylase Antibody, 1-4 alpha D glucan maltohydrolase antibody, 14 alpha D glucan maltohydrolase antibody, AMYB antibody, BMY1 antibody

Purification: Anti-Beta Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Beta Amylase [Sweet Potato]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Beta Amylase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of b Amylase [Sweet Potato] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for beta-Amylase 1

Target: beta-Amylase 1 (BAM1)

Alternative Name: BMY1, AMYB

Background: Background: Beta Amylase has catalytic activity of hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.

UniProt: P10537