ATP6V0B antibody (Middle Region)

Catalog No. ABIN950566

This Rabbit Polyclonal antibody specifically detects ATP6V0B in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for ATP6V0B antibody (Middle Region) (ABIN950566)

Target: ATP6V0B (ATPase, H+ Transporting, Lysosomal 21kDa, V0 Subunit B (ATP6V0B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V0B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ATP6V0B Antibody

Binding Specificity: AA 111-139, Middle Region

Specificity: This antibody reacts to ATP6V0B.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography on Protein A

Immunogen: KLH conjugated synthetic peptide between 111-139 amino acids from the Central region of human ATP6V0B

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ATP6V0B

Target: ATP6V0B (ATPase, H+ Transporting, Lysosomal 21kDa, V0 Subunit B (ATP6V0B))

Alternative Name: ATP6V0B

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is part of the transmembrane V0 domain and is the human counterpart of yeast VMA16. Two alternatively spliced transcript variants that encode different proteins have been found for this gene.Synonyms: ATP6F, HATPL, V-ATPase 21 kDa proteolipid subunit, V-type proton ATPase 21 kDa proteolipid subunit, Vacuolar proton pump 21 kDa proteolipid subunit

Gene ID: 533

NCBI Accession: NP_001034546

Pathways: Transition Metal Ion Homeostasis, Proton Transport