CD247 antibody (AA 151-164) (FITC)

Catalog No. ABIN951240

The Hamster Monoclonal anti-CD247 antibody has been validated for FACS and IF. It is suitable to detect CD247 in samples from Human and Mouse.

Quick Overview for CD247 antibody (AA 151-164) (FITC) (ABIN951240)

Target: CD247 (CD247 Molecule (CD247))

Reactivity: Human, Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD247 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: H146-968

Product Details anti-CD247 Antibody (FITC)

Binding Specificity: AA 151-164

Specificity: The antibody reacts with CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Characteristics: Synonyms: T3Z, TCRZ, CD3 zeta chain, T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3zeta chain

Immunogen: Synthetic peptide corresponding to amino acids 151-164 of Mouse CD3 zeta

Isotype: IgG

Application Details

Application Notes: Flow Cytometry: 2 μg/mL (starting point).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD247

Target: CD247 (CD247 Molecule (CD247))

Alternative Name: CD247 / CD3Z

Background: CD3 complex is crucial in transducing antigen-recognition signals into the cytopasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.Synonyms: CD3 zeta chain, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain, T3Z, TCRZ

Gene ID: 12503

UniProt: P24161

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway