CD28 antibody (FITC)

Catalog No. ABIN951273

This anti-CD28 antibody is a Mouse Monoclonal antibody detecting CD28 in FACS. Suitable for Rat.

Quick Overview for CD28 antibody (FITC) (ABIN951273)

Target: CD28

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: JJ319

Product Details anti-CD28 Antibody (FITC)

Specificity: Anti-rat CD28 monoclonal antibody recognizes a 90 kDa homeodimeric cell surface glycoprotein. CD28 has been found to be a potent costimulatory receptor on T cells. It is expressed on all peripheral rat ab and most gd T cells, as well as on approximately half of all NK cells. This clone can costimulate T cell proliferation and IL-2 secretion by resting rat T cells.

Purification: Affinity chromatography on Protein G

Immunogen: Rat CD28 transfected A20J cells Donor: BALB/c spleen Fusion Partner: X63-Ag 8.653

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0,1 mg/mL

Buffer: PBS containing 0.02 % sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted at -20 °C for longer. Should this product contain a precipitate we recommend microcentrifugation before use.

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: Synonyms: T-cell-specific surface glycoprotein CD28, TP44

Gene ID: 25660

NCBI Accession: NP_037253

UniProt: P31042

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response