CD55 antibody (Biotin)

Catalog No. ABIN951353

The Hamster Monoclonal anti-CD55 antibody has been validated for WB, FACS and EIA. It is suitable to detect CD55 in samples from Mouse.

Quick Overview for CD55 antibody (Biotin) (ABIN951353)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD55 antibody is conjugated to Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Clone: RIKO-3

Product Details anti-CD55 Antibody (Biotin)

Specificity: This DAF Monoclonal Antibody (Clone: RIKO-3) detects Mouse DAF

Purification: Protein G Affinity Chromatography.

Immunogen: GPI-DAF transfected CHO cells from Armenian Hamster spleen. Fusion Partner: P3U1

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55 / DAF

Background: DAF is one of several membrane proteins that prevent host cells from homologous complement attack. The two mouse DAF isoforms are effective against rat complement, but not against human and guinea pig complement. GPI-DAF is expressed on erythrocytes, spleen and testis while TM-DAF (transmembrane-DAF) is expressed only in testis. Both types of DAF transfectants avoided C3 deposition more successfully than non-transfectant cells.Synonyms: Complement decay-accelerating factor

Gene ID: 13136

NCBI Accession: NP_034146

UniProt: Q61475

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity