CD73 antibody

Catalog No. ABIN951383

The Mouse Monoclonal anti-CD73 antibody has been validated for FACS. It is suitable to detect CD73 in samples from Human.

Quick Overview for CD73 antibody (ABIN951383)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD73 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: AD2

Product Details anti-CD73 Antibody

Specificity: This antibody recognizes CD73, a 70 kDa GPI-anchored 5´-nucleotidase expressed predominantly on T and B cell subsets, follicular dendritic cells and endothelial cells.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein A affinity chromatography

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: CD73

Background: CD73 (ecto-5´-nucleotidase) is a 70 kDa glycoprotein anchored to the extracellular leaflet of the plasma membrane by GPI. This ecto-enzyme catalyzes dephosphorylation of AMP to adenosine. CD73 is expressed in various types of cells, such as epithelial, muscle, and endothelial cells, neutrophils, lymphocytes and fibroblasts. Inflammatory mediators support CD73 expression and its enzymatic activity, leading to the release of adenosine, which modulates inflammation through adenosine receptors. CD73 is expressed in a variety of lymphomas and leukemias, including ALL and CLL, whereas immunodeficient patients usually express low levels of this protein.Synonyms: 5'-nucleotidase, NT5, NT5E, NTE

Gene ID: 4907

NCBI Accession: NP_001191742

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process