F12 antibody (N-Term)

Catalog No. ABIN951608

The Rabbit Polyclonal anti-F12 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect F12 in samples from Human.

Quick Overview for F12 antibody (N-Term) (ABIN951608)

Target: F12 (Coagulation Factor XII (F12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This F12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-F12 Antibody

Binding Specificity: AA 13-43, N-Term

Specificity: This antibody recognizes Human Coagulation factor XII (F12) (N-term).

Purification: Affinity Chromatography on Protein A

Immunogen: KLH conjugated synthetic peptide between 13~43 amino acids from the N-terminal region of human F12

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for F12

Target: F12 (Coagulation Factor XII (F12))

Background: This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI.Synonyms: HAF, Hageman factor

Molecular Weight: 67818 Da

Gene ID: 2161

NCBI Accession: NP_000496

Pathways: Complement System