BTRC antibody (N-Term)

Catalog No. ABIN952275

This anti-BTRC antibody is a Rabbit Polyclonal antibody detecting BTRC in WB, IF, IHC (p) and EIA. Suitable for Human.

Quick Overview for BTRC antibody (N-Term) (ABIN952275)

Target: BTRC (beta-Transducin Repeat Containing (BTRC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BTRC antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-BTRC Antibody

Binding Specificity: AA 24-60, N-Term

Specificity: This antibody reacts to FBW1A.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography on Protein A

Immunogen: KLH conjugated synthetic peptide between 24-60 amino acids from the N-terminal region of human BRTC1

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for BTRC

Target: BTRC (beta-Transducin Repeat Containing (BTRC))

Alternative Name: FBW1A

Background: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class, in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway.Synonyms: BTRC, BTRCP, E3RSIkappaB, F-box and WD repeats protein beta-TrCP, F-box/WD repeat-containing protein 1A, FBXW1A, pIkappaBalpha-E3 receptor subunit

Gene ID: 8945

NCBI Accession: NP_003930

Pathways: Cell Division Cycle, Hedgehog Signaling