GFAP antibody

Catalog No. ABIN952496

The Chicken Polyclonal anti-GFAP antibody has been validated for WB, IF and IHC (p). It is suitable to detect GFAP in samples from Human, Rat and Mouse. There are 7+ publications available.

Quick Overview for GFAP antibody (ABIN952496)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Mouse

Host: Chicken

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GFAP Antibody

Specificity: Recognizes Glial Fibrillary Acidic Protein (GFAP).

Purification: Affinity Chromatography using a peptide column.

Immunogen: Recombinant Human GFAP (expressed in bacteria). After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Buffer: 10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted in the dark at 2-8 °C.

References for anti-GFAP antibody (ABIN952496)

Venables, Lapasset, Gadea, Fort, Klinck, Irimia, Vignal, Thibault, Prinos, Chabot, Abou Elela, Roux, Lemaitre, Tazi: "MBNL1 and RBFOX2 cooperate to establish a splicing programme involved in pluripotent stem cell differentiation." in: Nature communications, Vol. 4, pp. 2480, (2014) (PubMed).

Schoppmann, Ricken, Ilhan-Mutlu, Nirtl, Streubel, Preusser, Birner: "Downregulation of CIC does not associate with overexpression of ETV1 or MAP kinase pathway activation in gastrointestinal stromal tumors." in: Cancer investigation, Vol. 32, Issue 7, pp. 363-7, (2014) (PubMed).

Fofana, Xiao, Thumann, Turek, Zona, Tawar, Grunert, Thompson, Zeisel, Baumert: "A novel monoclonal anti-CD81 antibody produced by genetic immunization efficiently inhibits Hepatitis C virus cell-cell transmission." in: PLoS ONE, Vol. 8, Issue 5, pp. e64221, (2013) (PubMed).

Heid, Rickelt, Zimbelmann, Winter, Schumacher, Dörflinger: "Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells." in: PLoS ONE, Vol. 8, Issue 5, pp. e63061, (2013) (PubMed).

Kanemaru, Saitoh: "High-yield expression of mouse Aos1-Uba2-fusion SUMO-activating enzyme, mAU, in a baculovirus-insect cell system." in: Bioscience, biotechnology, and biochemistry, Vol. 77, Issue 7, pp. 1575-8, (2013) (PubMed).

Su, Chao, Huang, Weng, Jeng, Lai: "Rab5 and class III phosphoinositide 3-kinase Vps34 are involved in hepatitis C virus NS4B-induced autophagy." in: Journal of virology, Vol. 85, Issue 20, pp. 10561-71, (2011) (PubMed).

Huang, Zhou, Saberwal, Konieczna, Horvath, Katsoulidis, Platanias, Eklund: "Interferon consensus sequence binding protein (ICSBP) decreases beta-catenin activity in myeloid cells by repressing GAS2 transcription." in: Molecular and cellular biology, Vol. 30, Issue 19, pp. 4575-94, (2010) (PubMed).

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background: Human GFAP is a 49,749 dalton protein (432 amino acids) expressed by astrocytes of the central nervous system. GFAP is an intermediate filament protein and acts as an intra-cellular structural component of the astrocytic cytoskeleton. During embryonic and fetal life, GFAP is also expressed by radial glial cells of the CNS. Rare mutations of the GFAP gene in humans result in Alexander's disease, one of the leukodystrophies.Synonyms: Glial Fibrillary Acidic Protein

Gene ID: 2670

NCBI Accession: NP_001229305