MBP antibody

Catalog No. ABIN953563

This Chicken Polyclonal antibody specifically detects MBP in WB and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MBP antibody (ABIN953563)

Target: MBP (Myelin Basic Protein (MBP))

Reactivity: Human, Mouse, Rat

Host: Chicken

Clonality: Polyclonal

Conjugate: This MBP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-MBP Antibody

Specificity: Specific for the ~18k & 22k myelin basic protein in Western blots of Rat brain homogenate.

Cross-Reactivity (Details): Species reactivity (tested):Human, Bovine, Mouse and Rat.

Purification: Ig Fraction

Immunogen: Three peptide sequences conserved in higher vertebrate MBP proteins.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C.

Target Details for MBP

Target: MBP (Myelin Basic Protein (MBP))

Alternative Name: Myelin Basic Protein

Background: Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago (Eylar et al., 1971). The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells (Marty et al., 2002). In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22 kDa and another at about 18 kDa.Synonyms: MBP, Myelin membrane encephalitogenic protein

Gene ID: 4155

NCBI Accession: NP_001020252

UniProt: P02686