Ascorbate Oxidase antibody

Catalog No. ABIN95384

Supplier Product No.: 200-4148-0100

This Rabbit Polyclonal antibody specifically detects Ascorbate Oxidase in ELISA, WB and IP. It exhibits reactivity toward Pumpkin.

Quick Overview for Ascorbate Oxidase antibody (ABIN95384)

Target: Ascorbate Oxidase (AO)

Reactivity: Pumpkin

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ascorbate Oxidase antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-Ascorbate Oxidase Antibody

Supplier Product No.: 200-4148-0100

Supplier: Rockland

Cross-Reactivity (Details): Cross reactivity against Ascorbate Oxidase from other sources is unknown.

Purity: Anti-ASCORBATE OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ascorbate Oxidase [cucurbita].

Endotoxin Level: Low Endotoxin : No

Immunogen: Ascorbate Oxidase [cucurbita]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: ELISA Dilution: 1:25.000 - 1:125.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:2.000 - 1:10.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Ascorbate Oxidase

Target: Ascorbate Oxidase (AO)

Alternative Name: Ascorbate Oxidase

Background: Synonyms: L-ascorbate oxidase Ascorbase ASO EC=1.10.3.3

UniProt: P24792