Prothrombin antibody

Catalog No. ABIN954323

The Mouse Monoclonal anti-Prothrombin antibody has been validated for WB and EIA. It is suitable to detect Prothrombin in samples from Human.

Quick Overview for Prothrombin antibody (ABIN954323)

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prothrombin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: CaPro-20

Product Details anti-Prothrombin Antibody

Specificity: This antibody is specific for Human Prothrombin (CaPro-20).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein G Chromatography of Ascitic fluid.

Immunogen: Human Prothrombin (CaPro-20) purified from plasma.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Prothrombin

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Alternative Name: Prothrombin (F2)

Background: Prothrombin (Factor 2 in the coagulation cascade) is proteolytically cleaved at two sites by Factor Xa to form thrombin (Factor IIa) in an important step of the coagulation cascade which ultimately results in the stemming of blood loss. Thrombin being the single most important constituent of the coagulation cascade in terms of its feedback activation roles. Prothrombin also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in Prothrombin lead to various forms of thrombosis and dysprothrombinemia. Prothrombin is a 72 kDa blood clotting glycoprotein formed by and stored in the Liver. Prothrombin is present in the blood plasma and is converted to the key blood clotting agent thrombin, when blood vessels are damaged. Factor VII, another important haemostasis protein can be converted to VIIa by Factor IXa or Thrombin.Synonyms: Coagulation factor II

Gene ID: 2147

NCBI Accession: NP_000497

UniProt: P00734

Pathways: Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling