S100A8 antibody (Biotin)

Catalog No. ABIN954652

This Mouse Monoclonal antibody specifically detects S100A8 in WB, IF, FACS and IHC (p). It exhibits reactivity toward Human.

Quick Overview for S100A8 antibody (Biotin) (ABIN954652)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CF-145

Product Details anti-S100A8 Antibody (Biotin)

Specificity: This antibody detects Calgranulin A (S100A8).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein G Chromatography of Ascitic fluid.

Immunogen: Purified granulocyte antigen of Human origin.

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: S100A8 / Calgranulin-A / MRP8

Background: Calgranulin A, also known as MRP-8 and S100A8 is a member of the S100 family of proteins containing 2 EF hand (alpha helix, turn, alpha helix structure) calcium binding motifs. S100 proteins are localized in the cytoplasm and /or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. MRP-8 (S100A8) forms a heterodimeric complex with Calgranulin B, (MRP-14, S100A9) in the cytosol of monocyte and neutrophil cell types circulating in peripheral blood. Calgranulin A is found in elevated levels in the serum of cystic fibrosis cases and is also expressed in the skin of patients with psoriasis, eczematous dermatitis and squamous cell carcinoma.Synonyms: CAGA, CFAG, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP-8, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, S100-A8, Urinary stone protein band A

Gene ID: 6279

NCBI Accession: NP_002955

UniProt: P05109

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins