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SP1 antibody (C-Term)

Name: SP1 antibody (C-Term)
SKU: ABIN954894
Price: 768 USD
Availability: InStock

SP1 Reactivity: Human WB, IF, IHC (p), FACS, EIA Host: Rabbit Polyclonal unconjugated

Western blot analysis in K562 cell line lysates (35ug/lane) using SP1 / TSFP1 Antibody (C-term). (SP1 antibody (C-Term))

Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram) using SP1 / TSFP1 Antibody (C-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodie. (SP1 antibody (C-Term))

FACS

Confocal immunofluorescent analysis with WiDr cells using SP1 / TSFP1 Antibody (C-term), followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (SP1 antibody (C-Term))

Immunohistochemistry analysis in human colon carcinoma (Formalin-fixed, Paraffin-embedded) using SP1 / TSFP1 Antibody (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. (SP1 antibody (C-Term))

IHC (p)

4 images

Catalog No. ABIN954894

Datasheet as PDF

Target See all SP1 Antibodies

SP1 (Sp1 Transcription Factor (SP1))
Binding Specificity
21

12

10

7

4

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 684-715, C-Term
Reactivity
88

60

39

11

8

7

6

5

5

5

4

2

2

2

1
Human
Host
90

13

3
Rabbit
Compatible Secondaries
Clonality
84

22
Polyclonal
Conjugate
72

4

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This SP1 antibody is un-conjugated
Application
91

46

45

37

21

16

14

13

11

8

4

3

3

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Specificity

This antibody recognizes SP1 / TSFP1 at C-term.

Cross-Reactivity (Details)

Species reactivity (tested):Human

Purification

Purified through a Protein A column followed by peptide affinity purification

Immunogen

Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 684-715aa) of human SP1 / TSFP1

Isotype

Ig Fraction

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SP1 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Format

Liquid

Concentration

0.25 mg/mL

Buffer

PBS with 0.09 % (W/V) Sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

SP1 (Sp1 Transcription Factor (SP1))

Abstract

SP1 Products

Background

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.Synonyms: Transcription factor Sp1

Gene ID

6667

NCBI Accession

NP_003100

Pathways

Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction