Topoisomerase II alpha antibody (C-Term)

Catalog No. ABIN955290

This anti-Topoisomerase II alpha antibody is a Rabbit Polyclonal antibody detecting Topoisomerase II alpha in WB, IF, IHC (p) and EIA. Suitable for Human.

Quick Overview for Topoisomerase II alpha antibody (C-Term) (ABIN955290)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: AA 1174-1204, C-Term

Specificity: This antibody detects TOP2A (C-term).

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein A column followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide between 1174~1204 amino acids from the C-terminal region of human TOP2A

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A

Background: TOP2A encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA.Synonyms: DNA topoisomerase 2-alpha, DNA topoisomerase II alpha isozyme, TOP2, Top-2

Gene ID: 7153

NCBI Accession: NP_001058

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases