PYM antibody (N-Term)

Catalog No. ABIN955577

The Rabbit Polyclonal anti-PYM antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect PYM in samples from Human and Mouse.

Quick Overview for PYM antibody (N-Term) (ABIN955577)

Target: PYM (WIBG) (Partner of Y14 and mago (WIBG))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PYM Antibody

Binding Specificity: AA 15-44, N-Term

Specificity: This antibody detects WIBG / PYM (N-term).

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse

Purification: Protein A column followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide between 15-44 amino acids from the N-terminal region of human WIBG

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer.

Target Details for PYM

Target: PYM (WIBG) (Partner of Y14 and mago (WIBG))

Alternative Name: WIBG / PYM

Background: Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmarks for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. Acts as a EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated. Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions. May bind RNA, the relevance of RNA-binding remains unclear in vivo, RNA-binding was detected by PubMed:14968132, while PubMed:19410547 did not detect RNA-binding activity independently of the EJC.Synonyms: Partner of Y14 and mago, Protein wibg homolog

Gene ID: 84305

NCBI Accession: NP_001137325