FZD6 antibody (Cysteine-Rich Domain, Extracellular)

Catalog No. ABIN958405

This Goat Polyclonal antibody specifically detects FZD6 in WB, ELISA, IHC, FACS and IHC (pfa). It exhibits reactivity toward Mouse.

Quick Overview for FZD6 antibody (Cysteine-Rich Domain, Extracellular) (ABIN958405)

Target: FZD6 (Frizzled Family Receptor 6 (FZD6))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FZD6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraformaldehyde) (IHC (pfa))

Product Details anti-FZD6 Antibody

Binding Specificity: Cysteine-Rich Domain, Extracellular

Specificity: Recognizes mouse Frizzled-6 extracellular Cysteine-Rich Domain (CRD). Less than 1 % cross-reactivity with rmFrizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-4, rmFrizzled-7, rmFrizzled-8 and rmFrizzled-9.

Purification: Immunoaffinity purified

Immunogen: NS0-derived rmFrizzled-6 extracellular Cysteine-Rich Domain (CRD).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo, IHC, IHC-Pfa (5 - 10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry. Between each step, cells were washed with 0.1 % BSA in PBS. Flow Cytometry: 0.1 mg/mL. Use 10 μL to label 1-2.5x10^5 cells in a total volume <200 μL. The binding of unlabeled primary antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of IgG (FITC) xGt.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 1 mL of Sterile PBS.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for FZD6

Target: FZD6 (Frizzled Family Receptor 6 (FZD6))

Alternative Name: FZD6 / Frizzled 6

Background: Name/Gene ID: FZD6
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD6, Frizzled family receptor 6, FZ-6, HFZ6, Frizzled 6, Frizzled-6, Frizzled homolog 6, FZ6, NDNC10

Gene ID: 8323

UniProt: O60353

Pathways: WNT Signaling, Tube Formation