Melanoma Associated Antigen antibody

Catalog No. ABIN959527

This Mouse Monoclonal antibody specifically detects Melanoma Associated Antigen in IHC, ELISA, IHC (fro), IF, IHC (p) and IP. It exhibits reactivity toward Human.

Quick Overview for Melanoma Associated Antigen antibody (ABIN959527)

Target: Melanoma Associated Antigen (MAGE)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Melanoma Associated Antigen antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: NKI-C3

Product Details anti-Melanoma Associated Antigen Antibody

Specificity: Used for diagnostic by staining of lysosomes/melanosomes. Directed against a predominantly intracellular protein of human melanoma cells. The antigen is abundantly present in malignant melanomas, nevocellular nevi, and neuroendocrine tumors, whereas it has not been detectable in most other tissues. The antigen recognized is a glycoprotein which appears as a broad band in SDS-PAGE. The antigen was shown to consist of a single protein backbone to which two or three V-linked glycans were added cotranslationally. Extensive further heterogeneity was generated in the Colgi compartmen and was shown to be dependent on the presence of complex type sugars. Although the antigen is associated with melanomas, it was not codistributed with the tyrosinase activity associated with melanogenesis. The antigen did show codistribution with cathepsin D, which is a marker for lysosomal functions.This antibody was originally described as a marker for melanoma. Recently, it resurfaced as a marker to separate cellular neurothekeoma from other dermal tumors in the differential diagnosis.

Purification: Purified

Immunogen: Melanoma-associated antigen

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IF, IHC, IHC-Fr, IHC-P (10 μg/mL), IP

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for Melanoma Associated Antigen

Target: Melanoma Associated Antigen (MAGE)

Alternative Name: Melanoma-Associated Antigen