CA2 antibody

Catalog No. ABIN96026

Supplier Product No.: 200-401-136-0100

Product Details anti-CA2 Antibody

Target: CA2 (Carbonic Anhydrase II (CA2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Supplier Product No.: 200-401-136-0100

Supplier: Rockland

Purpose: Carbonic Anhydrase II Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Human Erythrocytes].

Characteristics: Synonyms: rabbit anti-Carbonic Anhydrase II Antibody, Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody, CAC, CA-II

Purification: Anti-Carbonic Anhydrase II Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Carbonic Anhydrase II Antibody was produced by repeated immunizations with human erythrocytes Carbonic Anhydrase II.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Carbonic Anhydrase II Antibody has been tested by ELISA, western blot, and immunohistochemistry. Expect ~29.2 kDa. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:2000 to 1:10,000

ELISA Dilution: 1:165,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: CA2 (CA2 Products)

Background: Background: Carbonic Anhydrase II Antibody detects Carbonic Anhydrase II. Carbonic Anhydrase II is essential for bone resorption and osteoclast differentiation by similarity. It can hydrate cyanamide to urea and is involved in the regulation of fluid secretion into the anterior chamber of the eye. Anti-Carbonic Anhydrase II Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene ID: 760

NCBI Accession: NP_000058

UniProt: P00918