KLF4 antibody (AA 80-129)

Catalog No. ABIN960992

The Rabbit Polyclonal anti-KLF4 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect KLF4 in samples from Human, Cow, Horse, Dog, Sheep, Pig and Monkey.

Quick Overview for KLF4 antibody (AA 80-129) (ABIN960992)

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Reactivity: Human, Cow, Horse, Dog, Sheep, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KLF4 Antibody

Binding Specificity: AA 80-129

Brand: IHC-plus™

Specificity: Human KLF4

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Dog, Horse (86%) Sheep, Bovine, Pig (80%).

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa80-129 of human KLF4 (O43474, NP_004226). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset (100%), Galago, Dog, Bat, Horse (86%), Sheep, Elephant, Panda, Cat, Bovine, Pig (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (2.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name: KLF4

Background: Name/Gene ID: KLF4

Synonyms: KLF4, EZF, GKLF, Kruppel-like factor 4 (gut), Hezf, Krueppel-like factor 4

Gene ID: 9314

NCBI Accession: NP_004226

UniProt: O43474

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance