CRYAB antibody (pSer59)

Catalog No. ABIN961132

This Rabbit Polyclonal antibody specifically detects CRYAB in WB and IP. It exhibits reactivity toward Human, Rat, Mouse, Cow, Pig, Sheep and Dog.

Quick Overview for CRYAB antibody (pSer59) (ABIN961132)

Target: CRYAB (Crystallin, alpha B (CRYAB))

Reactivity: Human, Rat, Mouse, Cow, Pig, Sheep, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRYAB antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-CRYAB Antibody

Binding Specificity: AA 54-64, pSer59

Specificity: This antibody detects an ~22kD protein, corresponding to the apparent molecular mass of phosphorylated alpha-B-crystallin on SDS-PAGE immunoblots, in samples from mouse, rat, bovine, canine, porcine and sheep. There is no reactivity with non-phosphorylated alpha B-crystallin. This antibody may cross react with beta crystallin.

Purification: Purified

Immunogen: Human synthetic peptide corresponding to amino acids 54-64 and containing phosphorylated serine 59. This peptide is conserved among mouse, rat and bovine.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IP (12.5 μg/mL), WB

Usage: Western Blot (Colorimetric): 2 μg/mL. Immunoprecipitation: 12.5 μg/mL. Positive control: Alpha Crystallin Protein.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.0, 3 % BSA, 0.1 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CRYAB

Target: CRYAB (Crystallin, alpha B (CRYAB))

Alternative Name: CRYAB / Alpha B Crystallin

Background: Name/Gene ID: CRYAB

Synonyms: CRYAB, Alpha-crystallin B chain, CRYA2, Crystallin, alpha B, CTPP2, Heat shock protein beta-5, HSPB5, Alpha B Crystallin, Alpha(B)-crystallin, Rosenthal fiber component, Heat-shock 20 kD like-protein

Gene ID: 1410

UniProt: P02511