Ephrin B antibody

Catalog No. ABIN962143

This Mouse Monoclonal antibody specifically detects Ephrin B in ELISA, WB and IP. It exhibits reactivity toward Mouse.

Quick Overview for Ephrin B antibody (ABIN962143)

Target: Ephrin B (EphB)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ephrin B antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-Ephrin B Antibody

Specificity: This antibody recognizes over-expressed and endogenous ephrin-B, as well as phosphorylated ephrin-B. On Western blots it identifies a band at ~50-60kD. Reactivity has been confirmed with human 293T and MCF10A lysates and chicken cell lysates. Based on amino acid sequence homology, cross-reactivity with mouse is expected.

Purification: Protein A purified

Immunogen: Synthetic peptide derived from the ephrin-B sequence.

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (1 - 3 μg/mL), IP (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Western Blot: 1-3 μg/mL. Immunoprecipitation: 5 μg/mL. ELISA: 1-3 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Ephrin B

Target: Ephrin B (EphB)

Background: Name/Gene ID: EphB

Synonyms: EphB