PKC zeta antibody (Internal Region)

Catalog No. ABIN962147

The Rabbit Polyclonal anti-PKC zeta antibody has been validated for WB and IP. It is suitable to detect PKC zeta in samples from Mouse.

Quick Overview for PKC zeta antibody (Internal Region) (ABIN962147)

Target: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC zeta antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-PKC zeta Antibody

Binding Specificity: Internal Region

Specificity: Reacts with the mouse PKCz (~70kD) and PKMz (~55kD) proteins. The brain-specific isoform of this protein (PKMz) may appear as a doublet at ~55kD in western blots of brain homogenates. In mouse kidney homogenate, it identifies the band at ~70kD. Reactivity has been confirmed with mouse kidney and brain homogenates.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the middle region of the catalytic domain of the mouse protein kinase C, zeta isoform (PKCz) protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation. Western Blot: 1-3 μg/mL. Immunoprecipitation: 7 μg/IP reaction.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for PKC zeta

Target: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Alternative Name: PRKCZ / PKC-Zeta

Background: Name/Gene ID: PRKCZ
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCZ, PKC-ZETA, PKC2, Pkcz, PKCzeta, Protein kinase c zeta, Protein kinase C zeta type, NPKC-zeta, PKC Zeta, Protein kinase C, zeta, Protein kinase Czeta

Gene ID: 5590

UniProt: Q05513

Pathways: NF-kappaB Signaling, RTK Signaling, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling