MYB antibody (pThr486)

Catalog No. ABIN964595

Supplier Product No.: 600-401-c47

The Rabbit Polyclonal anti-MYB antibody has been validated for WB and ELISA. It is suitable to detect MYB in samples from Mouse.

Quick Overview for MYB antibody (pThr486) (ABIN964595)

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MYB Antibody

Binding Specificity: pThr486

Supplier Product No.: 600-401-c47

Supplier: Rockland

Purpose: C-Myb phosphoT486 Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against the phosphorylated form of mouse c-Myb protein at the pT486 residue.

Characteristics: Synonyms: rabbit anti-c-Myb pT486 Antibody, Proto-oncogene c-Myb antibody, C myb antibody, MYB antibody, Transcriptional activator Myb, cmyb antibody

Purification: Antiserum was first purified against the phosphorylated form of the immunizing peptide.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse c-Myb protein surrounding amino acid residue 486.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting using M1 cells which express endogenous wild-type c-Myb protein and in COS7 cells transfected with c-Myb.  Specific conditions for reactivity and detection of phosphorylated c-Myb should be optimized by the end user.  Expect a band approximately ~75 kDa in size corresponding to phosphorylated c-Myb by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:2,500

ELISA Dilution: 1:5,000-1:16,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.91 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MYB

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Alternative Name: Myb

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The transcription factor c-Myb plays an important role in the development of all hematopoietic lineages during definitive hematopoiesis. Its critical role in hematopoiesis has been first demonstrated in experiments with targeted disruption of the c-Myb gene where deletion of both alleles of the c-Myb gene was embryonally lethal due to severe anemia. c-Myb is a short-lived sequence specific DNA-binding protein that regulates transcription of several important genes involved directly in cellular processes such as proliferation, differentiation, and apoptosis. Post-translational modifications such as phosphorylation, acetylation, ubiquitination, and conjugation of SUMO proteins are crucial for modulation of the transactivation activity of c-Myb. Several phosphorylation sites serve to regulate c-Myb activity.

Gene ID: 17863, 110556654

UniProt: P06876

Pathways: Chromatin Binding, Positive Regulation of Immune Effector Process