YBX1 antibody

Catalog No. ABIN964624

Supplier Product No.: 200-401-c16

Product Details anti-YBX1 Antibody

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Reactivity: Borrelia burgdorferi

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YBX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 200-401-c16

Supplier: Rockland

Purpose: DbpB Antibody

Cross-Reactivity (Details): This antibody is specific for Borrelia burgdorferi DbpB protein.

Characteristics: Synonyms: rabbit anti-DbpB Antibody, Decorin-binding protein B, Borrelia burgdorferi DbpB, dbp-B, dbp B

Purification: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.

Immunogen:

Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi Dbp-B protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 17.9 kDa in size corresponding to Borrelia burgdorferi DbpB protein by Western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for YBX1

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Alternative Name: DbpB (YBX1 Products)

Background: Background: Decorin-binding protein B, or DbpB, binds to decorin, which may mediate the adherence of B.burgdorferi to collagen fibers in skin and other tissues. Spirochetal surface adhesions mediate attachment to decorin, a major component of the host extracellular matrix enabling bacteria to colonize in mammalian tissues. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host. Borrelia burgdorferi can colonize multiple tissues, and is capable of attachment to diverse cell types. The expression of decorin-binding protein (Dbp) A and/or DbpB, two B. burgdorferi surface proteins that bind GAGs, is sufficient to convert a high-passage nonadherent B. burgdorferi strain into one that efficiently binds 293 epithelial cells.

Gene ID: 1194341

NCBI Accession: WP_010890381

UniProt: P0CL68

Pathways: Regulation of Muscle Cell Differentiation