TEV Protease antibody

Catalog No. ABIN964715

Supplier Product No.: 200-401-b91

The Rabbit Polyclonal anti-TEV Protease antibody is suitable to detect TEV Protease in samples from Tobacco Etch Virus (TEV). It has been validated for ELISA and WB.

Quick Overview for TEV Protease antibody (ABIN964715)

Target: TEV Protease

Reactivity: Tobacco Etch Virus (TEV)

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB)

Product Details

Supplier Product No.: 200-401-b91

Supplier: Rockland

Purpose: TEV Protease Antibody

Cross-Reactivity (Details): Assay by western blot showed no reactivity to MBP.

Characteristics: Synonyms: rabbit anti-TEV Protease Antibody, Tobacco Etch Virus, tobacco etch potyvirus, TEV, TEV Protease

Purification: This product was protein A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant MBP-and-poly-His-tagged auto inactivation-resistant mutant TEV Protease.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This protein A purified antibody has been tested for use in western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 27 kDa in size corresponding to TEV by western blotting in the appropriate cell lysate or extract. Anti-TEV protease may be useful to detect residual TEV protease in preparations of recombinant proteins in which that protease may interfere with downstream manipulations.

Western Blot Dilution: 1:500

ELISA Dilution: 1:1,500-1:27,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: TEV Protease

Target Type: Viral Protein

Background: Background: TEV protease, encoded by the Tobacco Etch Virus (TEV), is a 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the virus (TEV). It is widely used for cleaving fusion proteins because of its sequence specificity. It recognizes a linear epitope of the general form E-Xaa-Xaa-Y-Xaa-Q-(G/S). Cleavage occurs between Q and G or Q and S. The structure of TEV protease is similar to that of the serine protease family. Like serine proteases, TEV protease utilizes a catalytic triad of residues to hydrolyze peptide bonds. The distinguishing feature of TEV protease, however, is that instead of the serine nucleophile in the triad Ser-Asp-His, there is a cysteine, which may explain the resistance of TEV protease to protease inhibitors which are commonly used. The strain used is the auto inactivation-resistant mutant S219V. The catalytic activity of the S219V mutant is approximately 2 fold greater than that of the wild-type protease.

Gene ID: 1502321

NCBI Accession: NP_734212

UniProt: P04517