IL-8 antibody

Catalog No. ABIN964737

Supplier Product No.: 202-401-311

This Rabbit Polyclonal antibody specifically detects IL-8 in WB and ELISA. It exhibits reactivity toward Cat.

Quick Overview for IL-8 antibody (ABIN964737)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivity: Cat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IL-8 Antibody

Supplier Product No.: 202-401-311

Supplier: Rockland

Purpose: IL-8 Antibody

Cross-Reactivity (Details): This antibody is specific for cat IL-8 protein.

Characteristics: Synonyms: IL8, rabbit anti-IL-8 antibody, rabbit anti-Interleukin-8 antibody, C-X-C motif chemokine 8, CXCL8, Chemokine (C-X-C motif) ligand 8

Purification: This product was protein A purified from monospecific antiserum by chromatography.

Immunogen:

Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to 79 amino acids of mature cat IL-8 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This protein A purified CXCL8 antibody has been tested by Western blotting and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 8.9 kDa in size corresponding to cat IL-8 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500-1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.13 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for IL-8

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: CXCL8

Background: Background: IL-8 (CXCL8) is a small cytokine belonging to the chemokine CXC subfamily. There have been 17 different CXC chemokines described in mammals. The members of the CXC chemokine subfamily have been subdivided into two categories - those with a specific amino acid sequence of glutamic acid-leucine-arginine (ELR) immediately before the first cysteine of the CXC motif (ELR-positive), and those without an ELR motif (ELR-negative). ELR-positive CXC chemokines specifically induce the migration of neutrophils, and interact with chemokine receptors CXCR1 and CXCR2. IL-8 is an ELR-positive CXC chemokine. The primary function of IL-8 is to recruit neutrophils to the site of inflammation. It also activates neutrophil functions and promotes angiogenesis. Anti-IL8 antibody is ideal for investigators involved in Cancer, Cardiovascular and Immunology research.

Gene ID: 493836

UniProt: Q9XSX5

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy