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CCL20 antibody

CCL20 Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN964750
Supplier Product No.: 209-401-b51
  • Target See all CCL20 Antibodies
    CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
    Reactivity
    • 63
    • 38
    • 10
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 49
    • 24
    • 4
    • 1
    • 1
    Rabbit
    Clonality
    • 50
    • 29
    Polyclonal
    Conjugate
    • 34
    • 11
    • 6
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CCL20 antibody is un-conjugated
    Application
    • 47
    • 26
    • 20
    • 13
    • 13
    • 12
    • 10
    • 8
    • 6
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Supplier Product No.
    209-401-b51
    Supplier
    Rockland
    Purpose
    MIP-3 alpha Antibody
    Cross-Reactivity (Details)
    In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human MIP-3α in cell supernatants and certain body fluids.
    Characteristics
    Synonyms: rabbit anti-MIP-3 alpha Antibody, rabbit anti-MIP-3a Antibody, MIP-3α , CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1
    Purification
    This Protein A purified antibody was heated to 56°C for 30 minutes.
    Immunogen

    Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-3alpha protein.

    Immunogen Type: Recombinant Protein

    Isotype
    IgG
    Top Product
    Discover our top product CCL20 Primary Antibody
  • Application Notes

    Application Note: This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/μl by the LAL method. By western blot a band approximately 11 kDa in size corresponding to native human MIP-3α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

    Neutralization Dilution: 1:400

    Western Blot Dilution: 1:500-1:2,000

    ELISA Dilution: 1:20,000-1:100,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: None
    Preservative
    Without preservative
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
    Alternative Name
    CCL20 (CCL20 Products)
    Background
    Background: MIP-3α (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3α inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3α also possesses antibacterial activity against E.coli and S.aureus. MIP-3α is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
    Gene ID
    6364, 4759076
    UniProt
    P78556
    Pathways
    The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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