CCL20 antibody

Catalog No. ABIN964750

Supplier Product No.: 209-401-b51

This Rabbit Polyclonal antibody specifically detects CCL20 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for CCL20 antibody (ABIN964750)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL20 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CCL20 Antibody

Supplier Product No.: 209-401-b51

Supplier: Rockland

Purpose: MIP-3 alpha Antibody

Cross-Reactivity (Details): In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human MIP-3α in cell supernatants and certain body fluids.

Characteristics: Synonyms: rabbit anti-MIP-3 alpha Antibody, rabbit anti-MIP-3a Antibody, MIP-3α , CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1

Purification: This Protein A purified antibody was heated to 56°C for 30 minutes.

Immunogen:

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-3alpha protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/μl by the LAL method. By western blot a band approximately 11 kDa in size corresponding to native human MIP-3α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Neutralization Dilution: 1:400

Western Blot Dilution: 1:500-1:2,000

ELISA Dilution: 1:20,000-1:100,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: CCL20

Background: Background: MIP-3α (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3α inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3α also possesses antibacterial activity against E.coli and S.aureus. MIP-3α is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

Gene ID: 6364, 4759076

UniProt: P78556

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection