CCL20 antibody
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- Target See all CCL20 Antibodies
- CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CCL20 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Supplier Product No.
- 209-401-b51
- Supplier
- Rockland
- Purpose
- MIP-3 alpha Antibody
- Cross-Reactivity (Details)
- In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human MIP-3α in cell supernatants and certain body fluids.
- Characteristics
- Synonyms: rabbit anti-MIP-3 alpha Antibody, rabbit anti-MIP-3a Antibody, MIP-3α , CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1
- Purification
- This Protein A purified antibody was heated to 56°C for 30 minutes.
- Immunogen
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Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-3alpha protein.
Immunogen Type: Recombinant Protein
- Isotype
- IgG
- Top Product
- Discover our top product CCL20 Primary Antibody
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- Application Notes
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Application Note: This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/μl by the LAL method. By western blot a band approximately 11 kDa in size corresponding to native human MIP-3α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Neutralization Dilution: 1:400
Western Blot Dilution: 1:500-1:2,000
ELISA Dilution: 1:20,000-1:100,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: None - Preservative
- Without preservative
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
- Alternative Name
- CCL20 (CCL20 Products)
- Background
- Background: MIP-3α (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3α inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3α also possesses antibacterial activity against E.coli and S.aureus. MIP-3α is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
- Gene ID
- 6364, 4759076
- UniProt
- P78556
- Pathways
- The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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