Rabbit anti-Cow IgG (F(ab')2 Region) Antibody (FITC)

Catalog No. ABIN964806

Supplier Product No.: 301-4204

Quick Overview for Rabbit anti-Cow IgG (F(ab')2 Region) Antibody (FITC) (ABIN964806)

Target: IgG

Fragment: F(ab')2 fragment

Clonality: Polyclonal

Conjugate: FITC

Application: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Isotype: IgG

Product Details

Binding Specificity: F(ab')2 Region

Reactivity: Cow

Host: Rabbit

Supplier Product No.: 301-4204

Supplier: Rockland

Purpose: F(ab')2 Bovine IgG F(ab')2 Antibody Fluorescein Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Bovine IgG, Bovine IgG F(ab')2  and Bovine Serum.  No reaction was observed against Bovine IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin.

Characteristics: F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Purification: This product is a F(ab')2  fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above.

Immunogen: Optional[Immunogen]: Bovine IgG F(ab')2 fragment

Application Details

Application Notes: Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: F(ab')2 Anti-Bovine IgG F(ab')2 Fluorescein Antibody generated in rabbit detects Bovine F(ab')2. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.