PRDM1 antibody (C-Term)

Catalog No. ABIN964913

Supplier Product No.: 600-401-b52

The Rabbit Polyclonal anti-PRDM1 antibody has been validated for WB, ELISA, IHC and IP. It is suitable to detect PRDM1 in samples from Human.

Quick Overview for PRDM1 antibody (C-Term) (ABIN964913)

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRDM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-PRDM1 Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-b52

Supplier: Rockland

Purpose: PRDM1 BLIMP1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human BLIMP1 protein.

Characteristics: Synonyms: rabbit anti-Blimp-1 antibody, rabbit anti-PRDM-1 antibody, anti-PRDM1/BLIMP1 antibody, PR domain zinc finger protein 1, PR domain-containing protein 1, Beta-interferon gene positive regulatory domain I-binding factor, BLIMP-1, Beta-lymphocyte-induced maturation protein 1, positive regulatory domain I-binding factor 1, PRDI-BF1, PRDI-binding factor 1, BLIMP1, BLIMP 1, PRDM1

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Blimp1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of human, mouse, and rat PRDM1/BLIMP1.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 5 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~88 kDa in size corresponding to BLIMP1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000

Immunoprecipitation Dilution: 5-10 μg/mL

ELISA Dilution: 1:20,000 - 1:40,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.15 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Blimp1 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for PRDM1

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Alternative Name: PRDM1

Background: Background: Anti-Blimp1 is ideal for Immunology, Epigenetic and Nuclear Signaling research. PRDM1/Blimp1 (PR domain zinc finger protein 1, PR domain-containing protein 1, Beta-interferon gene positive regulatory domain I-binding factor, BLIMP-1, Positive regulatory domain I-binding factor 1, PRDI-binding factor 1, or PRDI-BF1, or Beta-lymphocyte-induced maturation protein 1) is one of several transcription factors which drives the maturation of B-lymphocytes into Ig secreting cells. In the nucleus, PRDM1/BLIMP1 functions as a transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene. Transcription of the PRDM1 gene is increased upon virus induction. PRDM1/Blimp1 also interacts with PRMT5. Two alternatively spliced transcript variants that encode different isoforms have been reported.

Gene ID: 639, 25008939

UniProt: Q60636

Pathways: Regulation of Muscle Cell Differentiation