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Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (Atto 647N) - Preadsorbed

WB, FLISA, FM Polyclonal IgG Atto 647N
Catalog No. ABIN964964
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2271
    • 457
    • 363
    • 188
    • 130
    • 123
    • 92
    • 29
    • 28
    • 28
    • 17
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    Heavy & Light Chain
    Reactivity
    • 684
    • 684
    • 567
    • 436
    • 378
    • 235
    • 199
    • 156
    • 138
    • 130
    • 113
    • 97
    • 95
    • 93
    • 82
    • 58
    • 44
    • 28
    • 27
    • 16
    • 12
    • 10
    • 8
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 1677
    • 1473
    • 546
    • 243
    • 104
    • 90
    • 33
    • 30
    • 21
    • 16
    • 15
    • 11
    • 11
    • 1
    Goat
    Clonality
    • 3419
    • 196
    • 1
    Polyclonal
    Conjugate
    • 578
    • 400
    • 393
    • 350
    • 276
    • 245
    • 191
    • 118
    • 98
    • 66
    • 64
    • 61
    • 58
    • 52
    • 48
    • 47
    • 44
    • 43
    • 43
    • 41
    • 39
    • 32
    • 32
    • 32
    • 32
    • 32
    • 32
    • 32
    • 31
    • 30
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 26
    • 26
    • 25
    • 21
    • 19
    • 17
    • 17
    • 16
    • 16
    • 15
    Atto 647N
    Application
    • 1778
    • 1700
    • 1687
    • 1440
    • 933
    • 909
    • 883
    • 463
    • 243
    • 243
    • 238
    • 100
    • 92
    • 85
    • 76
    • 50
    • 48
    • 32
    • 31
    • 27
    • 24
    • 22
    • 21
    • 16
    • 11
    • 8
    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
    Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.
    Characteristics
    Anti-Mouse IgG (H&L) conjugated to ATTO 647N is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Purification
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Mouse IgG whole molecule

    Isotype
    IgG
    Labeling Ratio
    1.5
  • Application Notes

    Application Note: Anti-Mouse IgG (H&L) conjugated to ATTO 647N is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

    FLISA Dilution: >1:20,000

    Western Blot Dilution: >1:10,000

    IF Microscopy Dilution: >1:5,000

    Comment

    The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 500 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Product is photosensitive and should be protected from light.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
    Expiry Date
    12 months
  • Fernandes, Loubens, Marinach, Coppée, Baron, Grand, Andre, Hamada, Langlois, Briquet, Bun, Silvie: "Plasmodium sporozoites require the protein B9 to invade hepatocytes." in: iScience, Vol. 26, Issue 2, pp. 106056, (2023) (PubMed).

    Maidorn, Olichon, Rizzoli, Opazo: "Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons." in: mAbs, Vol. 11, Issue 2, pp. 305-321, (2019) (PubMed).

    Richter, Wildhagen, Helm, Ußling, Schikorski, Rizzoli: "Comparative synaptosome imaging: a semi-quantitative method to obtain copy numbers for synaptic and neuronal proteins." in: Scientific reports, Vol. 8, Issue 1, pp. 14838, (2018) (PubMed).

    Purkey, Woolfrey, Crosby, Stich, Chick, Aoto, DellAcqua: "AKAP150 Palmitoylation Regulates Synaptic Incorporation of Ca2+-Permeable AMPA Receptors to Control LTP." in: Cell reports, Vol. 25, Issue 4, pp. 974-987.e4, (2018) (PubMed).

    Gomes de Castro, Höbartner, Opazo: "Aptamers provide superior stainings of cellular receptors studied under super-resolution microscopy." in: PLoS ONE, Vol. 12, Issue 2, pp. e0173050, (2017) (PubMed).

  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background

    Synonyms: Goat Anti-Mouse IgG Antibody ATTO647N Conjugation, Goat Anti-Mouse IgG ATTO 647N Conjugated Antibody

    Background: Anti-Mouse IgG ATTO 647N Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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