Rabbit anti-Chicken IgG (Heavy & Light Chain) Antibody (PE)

Catalog No. ABIN965028

Supplier Product No.: 703-408-002

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: Heavy & Light Chain

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: PE

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Supplier Product No.: 703-408-002

Supplier: Rockland

Purpose: F(ab')2 Chicken IgG (H&L) Antibody Phycoerythrin conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Chicken IgG and Chicken Serum.  No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).

Characteristics: F(ab')2 Anti-Chicken IgG Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation.  Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody.

Immunogen: Optional[Immunogen]: Chicken IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:100 - 1:250 IF Microscopy Dilution: 1:100 - 1:250 Other: FLOW 1:100 - 1:250

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.