alpha 2 Macroglobulin antibody (C-Term)

Catalog No. ABIN965492

The Rabbit Polyclonal anti-alpha 2 Macroglobulin antibody has been validated for IHC. It is suitable to detect alpha 2 Macroglobulin in samples from Human and Rat. There are 5+ publications available.

Quick Overview for alpha 2 Macroglobulin antibody (C-Term) (ABIN965492)

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha 2 Macroglobulin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-alpha 2 Macroglobulin Antibody

Binding Specificity: C-Term

Purification: Purified by antigen-specific affinity chromatography.

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to C-terminal residues of human A2M (Alpha-2-macroglobulin precursor)

Application Details

Application Notes: ELISA, Western blotting: 1µg/ml for 2hrs.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: This antibody is stored in PBS, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-alpha 2 Macroglobulin antibody (ABIN965492)

Zhang, Li, Martin, Aebersold: "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry." in: Nature biotechnology, Vol. 21, Issue 6, pp. 660-6, (2003) (PubMed).

Matthijs, Devriendt, Cassiman, Van den Berghe, Marynen: "Structure of the human alpha-2 macroglobulin gene and its promotor." in: Biochemical and biophysical research communications, Vol. 184, Issue 2, pp. 596-603, (1992) (PubMed).

Bishop, Bell: "Assembly of the endoplasmic reticulum phospholipid bilayer: the phosphatidylcholine transporter." in: Cell, Vol. 42, Issue 1, pp. 51-60, (1985) (PubMed).

Sottrup-Jensen, Stepanik, Kristensen, Wierzbicki, Jones, Lønblad, Magnusson, Petersen: "Primary structure of human alpha 2-macroglobulin. V. The complete structure." in: The Journal of biological chemistry, Vol. 259, Issue 13, pp. 8318-27, (1984) (PubMed).

Sottrup-Jensen, Lønblad, Stepanik, Petersen, Magnusson, Jörnvall: "Primary structure of the 'bait' region for proteinases in alpha 2-macroglobulin. Nature of the complex." in: FEBS letters, Vol. 127, Issue 2, pp. 167-73, (1981) (PubMed).

Target Details for alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Alternative Name: A2M

Background: A2M (Alpha-2-macroglobulin) is able to inhibit all four classes of proteinases by a unique `trapping' mechanism. This protein has a peptide stretch, called the `bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thiolester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Pathways: Lipid Metabolism