APOB antibody

Catalog No. ABIN965573

The Mouse Monoclonal anti-APOB antibody has been validated for WB, ELISA, FACS and IF. It is suitable to detect APOB in samples from Human. There is 1 publication available.

Quick Overview for APOB antibody (ABIN965573)

Target: APOB (Apolipoprotein B (APOB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APOB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 6G6

Product Details anti-APOB Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human ApoB expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-APOB antibody (ABIN965573)

Peterson, Mack, Hall, Alsup, Alexander, Sully, Sawires, Cheung, Otto, Gresham: "Apolipoprotein B Is an innate barrier against invasive Staphylococcus aureus infection." in: Cell host & microbe, Vol. 4, Issue 6, pp. 555-66, (2008) (PubMed).

Target Details for APOB

Target: APOB (Apolipoprotein B (APOB))

Alternative Name: ApoB

Background: This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.
Synonyms: FLDB, LDLCQ4

Molecular Weight: 516kDa

Gene ID: 338

Pathways: Lipid Metabolism